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Palaeoproteomics of bird bones for taxonomic classification (1 Viewer)

Fred Ruhe

Well-known member
Netherlands
IVO R. HORN, YVO KENENS, N. MAGNUS PALMBLAD, SUZANNE J. VAN DER PLAS-DUIVESTEIJN, BRAM W. LANGEVELD, HANNEKE J. M. MEIJER, HANS DALEBOUT, ROB J. MARISSEN, , ANJA FISCHER, F. B. VINCENT FLORENS, JONAS NIEMANN, KENNETH F. RIJSDIJK, ANNE S. SCHULP, JEROEN F. J. LAROS and BARBARA GRAVENDEEL, 2019

Palaeoproteomics of bird bones for taxonomic classification

Zoological Journal of the Linnean Society, 2019, XX, 1–16

Free pdf https://watermark.silverchair.com/z...g_OnV_gaTdt3lnMNHOeMc-qEDSPnXi1trW0-DROMr-tp4

Abstract:

We used proteomic profiling to taxonomically classify extinct, alongside extant bird species using mass spectrometry on ancient bone-derived collagen chains COL1A1 and COL1A2. Proteins of Holocene and Late Pleistocene-aged bones from dodo (Raphus cucullatus) and great auk (Pinguinus impennis), as well as bones from chicken (Gallus gallus), rock dove (Columba livia), zebra finch (Taeniopygia guttata) and peregrine falcon (Falco peregrinus), of various ages ranging from the present to 1455 years old were analysed. HCl and guandine-HCL-based protein extractions from fresh bone materials yielded up to 60% coverage of collagens COL1A1 and COL1A2, and extractions from ancient materials yielded up to 46% coverage of collagens COL1A1 and COL1A2. Data were retrieved from multiple peptide sequences obtained from different specimens and multiple extractions. Upon alignment, and in line with the latest evolutionary insights, protein data obtained from great auk grouped with data from a recently sequenced razorbill (Alca torda) genome. Similarly, protein data obtained from bones of dodo and modern rock dove grouped in a single clade. Lastly, protein data obtained from chicken bones, both from ancient and fresh materials, grouped as a separate, basal clade. Our proteomic analyses enabled taxonomic classification of all ancient bones, thereby complementing phylogenetics based on DNA.

Enjoy,

Fred
 
Last edited:

l_raty

laurent raty
This not a persistent address. (Clicking the link just yields a message saying: "Your session has timed out. Please go back to the article page and click the PDF link again.")

Article: https://doi.org/10.1093/zoolinnean/zlz012
Current direct access to pdf: https://academic.oup.com/zoolinnean...10.1093/zoolinnean/zlz012/28459833/zlz012.pdf (This will presumably become obsolete when the paper ceases to be an "advance article".)

Groetjes, L -
 

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