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Message: <blockquote data-quote="Surveyor" data-source="post: 1390645" data-attributes="member: 50720">Henry, the problem with exact matches of the RGB numbers is that I am averaging an area to get the value and even trying to pick the same area (in the above case I used 50x50 pixels) you will get a difference of a couple of counts. I do not have the time at the moment to redo the last Slide you posted, but I did crop an area out of the Nikon, Zeiss and Background of the last slide and process with the online software I listed in Post #17 (since this is an online script, I think it will work with Macs). I cropped a large section of each patch to a separate jpg and loaded that into the program. Look at the top three rows, the h, s and v rows and, also, notice the color wheel plot on the left of the page. Note how when sampling the patch the RGB values range in color and amplitude of various widths. The upper left box with a saturation value directly below shows the average hue. The total deviation from white is the fourth row, HSV value. The RGB values are below that, but it is hard for me to visualize them. At the moment, I do not see this as much of a factor since we are looking for a shift from the background to the inter-optic patch. When you did that last manipulation, it really brought out the red and green, at least on my monitor.</blockquote>