BirdForum is the net's largest birding community dedicated to wild birds and birding, and is absolutely FREE!

Register for an account to take part in lively discussions in the forum, post your pictures in the gallery and more.

Reply to thread

Message: <blockquote data-quote="Richard Klim" data-source="post: 1584223" data-attributes="member: 773">Manthey & Spellman 2009Now 6 clades...Manthey, J D & Spellman, G M 2009. Nuclear phylogeography of the brown creeper (Certhia americana) in pine/oak woodlands. 127th Stated Meeting of the AOU: Philadelphia, 2009.Abstract:"The brown creeper (Certhia americana) is a resident species of several regional pine-oak woodlands, making it a good model for a biogeographic study. Preliminary study of mitochondrial DNA variation (ND2 gene) identified six clades of C. americana, which correspond geographically with regional pine-oak woodlands. Mitochondrial phylogeographic patterns will be assessed by checking for congruence (or lack thereof) with nuclear phylogeographic patterns. The nuclear phylogeographic study will focus on sequence data from fifteen known nuclear loci supplemented by sequence data from fifteen anonymous nuclear loci, thereby reducing ascertainment bias. One (GMS2724) individual was used to construct a small-insert genomic library to produce genetic markers. From this library, polymerase chain reaction primers were designed if the fragment was not similar to any sequences in GenBank (for anonymous loci). Amplicons of designed primers were checked against Taeniopygia guttata genome information for general chromosome location. Loci were chosen from a wide-range of chromosomes, with GC content ranging from 37-63%. Preliminary analyses of the selected loci suggest that they will provide genetically informative data for further analysis."Richard</blockquote>

Verification